Leica VT1200S Manuel d'utilisateur télécharger pdf (Page 18)

Operating Manual V1.1 – 05/2007

5. Working with the Instrument

5.1 Description of the Typical Application

The Leica VT1200 / VT1200S is a microtome with a vibrating blade and is

predominately used for sectioning fixed and unfixed specimens during neu-

rological research.

• To simplify the process of inserting the spec-

imen, you can quickly move the specimen

receptacle to the lowest position by pressing

the DOWN button.

• To prepare high-quality sections, especially

for unfixed tissue, Leica suggests using the

optional VibroCheck measuring instrument to

determine the height amplitude of the blade

after each blade change, and then to minimize

it using the setting screw on the blade holder.

To perform the aforementioned process, in-

stall the VibroCheck instrument (see p. 36 for

the VT1200 or p. 38 for the VT1200S), install

the blade and adjust the clearance angle to

the desired position. Then take the measure-

ment and make the appropriate adjustments

to the placement of the blade holder.

Remove the VibroCheck according to the in-

structions, and turn the blade 90° toward the

top to ensure that the ice bath and buffer tray

can be installed safely.

• Insert the buffer tray into the ice bath and

cover it with the lid. Fill the ice bath with

crushed ice.

• Remove the cover and fill the buffer tray with

pre-cooled physiological buffer.

• Push the ice bath and buffer tray onto the

dovetail guide and clamp them down.

• Use cyanoacrylate adhesive to adhere the

specimen to the specimen plate and use a

manipulator to place it in the buffer tray.

Insert the hose for gassing the buffer into the

hose clamp.

• Use the UP button to lift the specimen to

the sectioning level of the blade quickly. Fine-

adjusting the blade-to-specimen proximity can

be performed using the desired step size 1, 10

or 100μm.

• Advance the blade toward the specimen us-

ing the "Blade forward" key.

• After sectioning the specimen, use the setting

dial to feed to the desired section thickness

using the selected step size (1, 10 or 100μm).

This raises the specimen receptacle to the

desired value.

• Start the sectioning process by pressing the

RUN/STOP key. The process can be stopped

again by pressing this key after the cut has

been completed. Use the "Blade back" key

to move the blade in front of the specimen.

Select the section thickness for the next cut

and start the sectioning process again.

• After completing the sectioning process,

remove the blade, dispose of the specimen

receptacle by pushing the DOWN key into its

lowest position, and unclamp, empty and clean

out the ice bath and buffer tray.

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Leica VT1200S Manuel d'utilisateur Page 18

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